Coding

Part:BBa_K118015:Design

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-10-06)


glgC coding sequence encoding ADP-glucose pyrophosphorylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genomic DNA sequence contains to EcoRI sites, both of which have been mutated out using the mutagenesis by blunt-ended ligation (MABEL) technique


Source

Escherichia coli JM109 genomic DNA.

References